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- 5-Me-dC-Brancher-CE Phosphoramidite
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5-Me-dC-CE Brancher Phosphoramidite (Brancher Molecule, BM) is used to construct comb-like oligonucleotides for use in signal amplification experiments or to create nanostructures. This is achieved by synthesising secondary oligonucleotides at branching points created by incorporating one or more BMs in the target primary sequence.
The BM is designed with three masked hydroxyl groups, its structure based on 5-Me-dC. The 3'-OH of the nucleoside is provided as the reactive phosphoramidite, whilst the 5'-OH has DMTr-protection. These two groups are utilised in the normal way to synthesise the primary oligonucleotide. The third OH is levulinyl-protected and remains so during oligo synthesis. This is easily deprotected after the primary synthesis to allow subsequent secondary oligo synthesis on the free OH.