PNAs are oligonucleotide analogues in which the standard sugar-phosphate backbone of DNA is substituted by a pseudo-peptide backbone. They were invented in 1991 by a group of scientists at the University of Copenhagen: Prof. Peter E. Nielsen, Prof. Ole Buchardt, Dr. Michael Egholm, and Dr. Rolf H. Berg (PNA Inventor Group). PNAs hybridise strongly and sequence-specifically to complementary DNA or RNA, however they are not degraded by nucleases or proteases. Such properties have made PNA the subject of great scientific interest and synthetic PNA oligomers have numerous identified and proposed applications in molecular biology procedures, diagnostic assays, as well as antisense and anti-gene therapies.
After extensive screening, the benzhydryloxycarbonyl (Bhoc) group was selected as the best choice for protecting the exocyclic amino groups of the nucleobases. This group provides sufficient protection during synthesis, is readily removed under the cleavage conditions, and renders solubility to the monomers. For PNA synthesis, therefore, we provide the four Fmoc/Bhoc monomers and a spacer molecule.
- Peptide Nucleic Acids (PNA)
- Fmoc-PNA-A(Bhoc)-OH
- Fmoc-PNA-C(Bhoc)-OH
- Fmoc-PNA-G(Bhoc)-OH
- Fmoc-PNA-T-OH
- Fmoc-AEEA-OH Spacer