Choosing a SynBase™ Solid Support
Link Technologies’ SynBase™ CPG solid supports are available in a variety of pore sizes and functionalised nucleoside loadings. Three pore sizes are offered: 500Å, 1000Å, and 3000Å. The 500Å support comes in a standard and high loading varieties (products are appended with either an 'S' or 'H' respectively). The range has been designed to offer optimum performance depending on the application, and the required nucleoside loading and length of sequence. The range is summarised below.
Shorter primer molecules (ca. 20 bases) should be synthesised on the 500Å support. Large-scale synthesis is most economical using the higher loading. Medium-length DNA (20-80 bases) for the widest range of applications, is best synthesised using the 1000Å support. Indeed, this support is the one we routinely use when preparing 3’-modifier supports.
For long sequences (>80 bases) we recommend the 3000Å support. This CPG has a formidable ability to allow synthesis of oligos up to 250 bases long. It is now also available in the catalogue as a Phosphate and Spacer CPG. Other 3'-modifier CPGs prepared with 3000Å support may be available as custom products so please contact us with your requirements.
Features Table
Product |
Average Pore Size (Å) |
Nominal Particle Size (µm) |
Nucleoside Loading (µmol/g) |
SynBase™ CPG 500/110 S |
500 |
110 |
30-60 |
SynBase™ CPG 500/110 H |
500 |
110 |
60-100 |
SynBase™ CPG 1000/110 |
1000 |
110 |
20-50 |
SynBase™ CPG 3000/110 |
3000 |
110 |
14-17 |
Optimum Use Table
<20 Bases |
20-80 Bases |
>80 Bases |
Larger-scale Synthesis |
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SynBase™ CPG 500/110 S |
• |
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SynBase™ CPG 500/110 H |
• |
• |
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SynBase™ CPG 1000/110 |
• |
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SynBase™ CPG 3000/110 |
• |