Use of O6-Bn-(dmf)dG-CE Phosphoramidite (2188) in Oligonucleotide Synthesis
Dissolution
Prepare a 0.1M in anhydrous acetonitrile (100μmol/1ml, 250mg/2.73ml).
Coupling
Recommended coupling time is 6min. It is recommended that fast deprotection amidites (e.g. Ac-dC, dmf-dG) are used for the standard bases in the oligonucleotide.
Deprotection
Use ammonia solution at room temperature for 3 days (from the reference below) or use AMA at RT.
Further Information
See: Novel synthesis of O6-alkylguanine containing oligodeoxyribonucleosides as substrates for the human DNA repair protein, O6-methylguanine DNA methyl transferase (MGMT), T.Shibata, N.Glynn, T.B.H.McMurry, R.S.McElhinney, G.P.Margison and D.M.Williams, Nucleic Acids Research, 1884-1891, 34, 2006. [full text]