Use of 2'-OMe-5-Me-N-Ac-C-CE Phosphoramidite (2192) in Oligonucleotide Synthesis
Dilution
Dilute in anhydrous acetonitrile to a concentration of 0.1M (250mg pack in 3.06ml, 100µmol pack (82mg) in 1ml).
Coupling
ETT, BTT or DCI can be used as activators. A coupling time of 6min (360s) is recommended.
Oxidation
No changes are required from standard methods.
Cleavage & Deprotection
Use AMA at 65°C for 10min.
Purification
All oligonucleotide purification methods are applicable..
Storage & Stability
Store in a freezer below -10°C. Diluted samples should be freshly prepared for use and used within 24h.