Modifications Incorporated in CpG Motifs of Oligodeoxynucleotides Lead to Antagonist Activity of Toll-like Receptors 7 and 9
D. Yu, D. Wang, F.-G. Zhu, L. Bhagat, M. Dai, E. R. Kandimalla and S. Agrawal, J. Med. Chem., 52, 5108-5114, 2009.
Oligodeoxynucleotides containing unmethylated CpG dinucleotides act as agonists of TLR9 and induce Th1-type immune responses. In this study, the authors synthesised CpG containing ODNs in which C or G was substituted with 2′-O-methylribonucleotides, 5-methyl-dC, or 2′-O-methyl-5-methyl-C and studied their immune stimulatory activity alone and in combination with TLR agonists.