Efficient In Vivo Delivery of siRNA to the Liver by Conjugation of α-Tocopherol
K. Nishina, T. Unno, Y. Uno, T. Kubodera, T. Kanouchi, H. Mizusawa and T. Yokota, Molecular Therapy, 16, 734-740, 2008.
In this study the authors used α-tocopherol (vitamin E), which has its own physiological transport pathway to most of the organs, as a carrier molecule of siRNA in vivo. The α-tocopherol was added to the antisense strand of 27/29-mer siRNA at the 5′-end using 5'-Tocopherol-CE Phosphoramidite (2163).