Use of 2'-OMe-I-CE Phosphoramidite (2098) in Oligonucleotide Synthesis
Dilution
Dilute in anhydrous acetonitrile to a concentration of 0.1M (250mg pack in 3.19ml, 100µmol pack (78.5mg) in 1ml).
Coupling
ETT, BTT or DCI can be used as activators. A coupling time of 6min (360s) is recommended.
Oxidation
No changes are required from standard methods.
Cleavage & Deprotection
No changes are required from standard oligonucleotide methods, but in general AMA at 65ºC for 10min is used.
Storage & Stability
Store in a freezer below -10°C. Diluted samples should be freshly prepared for use and used within 24h.