Use of 5'-Octyl-tocopherol-CE Phosphoramidite (2194) in Oligonucleotide Synthesis
Dilution
Dilute in anhydrous, alcohol-free DCM to a concentration of 0.1M (250mg pack in 3.29ml, 100µmol pack (76mg) in 1ml).
Coupling
ETT, BTT or DCI can be used as activators. A coupling time of 15min (900s) is recommended.
Oxidation
No changes are required from standard methods.
Cleavage & Deprotection
Use AMA at room temperature for 2h.
Purification
The modified oligonucleotide will be hydrophobic and will require a gradient that reaches at least 95% MeCN during HPLC purification to elute the product from a C18 RP-HPLC column.
Storage & Stability
Store in a freezer below -10°C. Diluted samples should be freshly prepared for use and used within 24h.